Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An epigenetic blueprint for placental syncytiotrophoblast development.

Placenta·2026
Same author

Amino Acid Restriction Impairs Human Endometrial Stromal Cell Decidualization and Is Rescued by Proline Supplementation.

Journal of cellular and molecular medicine·2025
Same author

Hypoxia and loss of GCM1 expression prevent differentiation and contact inhibition in human trophoblast stem cells.

Stem cell reports·2025
Same author

Maternal immune activation elicits rapid and sex-dependent changes in gene expression and vascular dysfunction in the rat placenta.

Placenta·2025
Same author

A stress paradox: the dual role of the unfolded protein response in the placenta.

Frontiers in endocrinology·2025
Same author

Decreased Incidence of Pediatric Neuro-Autoimmune Disorders During COVID-19 Pandemic Restrictions.

Journal of child neurology·2024

Related Experiment Video

Updated: Jul 9, 2025

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
10:28

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Published on: January 8, 2012

24.0K

Analyzing Trophoblast Fusion Using Immunofluorescence and Split Protein Complementation Assays.

Adam Jaremek1, Stephen J Renaud2,3,4

  • 1Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2023
PubMed
Summary

Researchers developed new methods to measure trophoblast fusion, crucial for placental development. These techniques improve the analysis of syncytiotrophoblast formation, aiding studies on factors regulating this process.

Keywords:
Cell fusionGreen fluorescent proteinImmunofluorescencePlacentaSplit protein complementationSyncytiotrophoblastTrophoblast

More Related Videos

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
08:26

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Published on: August 31, 2016

7.9K
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

14.1K

Related Experiment Videos

Last Updated: Jul 9, 2025

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
10:28

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Published on: January 8, 2012

24.0K
Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
08:26

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Published on: August 31, 2016

7.9K
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

14.1K

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Developmental Biology

Background:

  • Syncytiotrophoblast formation is vital for placental development.
  • Accurate measurement of trophoblast fusion is essential for research in this area.
  • Existing methods for analyzing trophoblast fusion have limitations.

Purpose of the Study:

  • To describe novel protocols for measuring trophoblast fusion efficiency.
  • To provide robust and accurate methods for analyzing syncytiotrophoblast formation in vitro.
  • To enhance the throughput of studies investigating factors regulating trophoblast fusion.

Main Methods:

  • Immunofluorescence assays to visualize and quantify trophoblast fusion.
  • A split green fluorescent protein (split-GFP) assay for detecting cell fusion.
  • Quantitative analysis of fusion index in cultured trophoblast cells.

Main Results:

  • The described protocols enable robust measurement of trophoblast fusion.
  • The split-GFP assay provides a sensitive and quantifiable signal upon cell fusion.
  • These methods allow for comprehensive analysis of fusion efficiency.

Conclusions:

  • The developed immunofluorescence and split-GFP assays offer accurate and high-throughput methods for studying trophoblast fusion.
  • These techniques can significantly advance research into the regulation of syncytiotrophoblast development.
  • Improved analytical tools are critical for understanding placental formation and function.