A Trojan Horse Approach Using Ustilago maydis to Study Apoplastic Maize (Zea mays) Peptides In Situ

Plant peptides are important signaling components in many parts of the plant lifecycle, e.g., development, reproduction, environmental stress response, and plant pathogen defenses. Yet, in maize, one of the most grown crops worldwide, only a few peptides have been identified and studied. In general, molecular research is severely impacted by time-consuming and costly maize transformation, and external application of purified peptides does not allow functional analysis in deeper cell layers due to the thickness of the tissue. In an attempt to bypass these problems while studying the function of small secreted proteins in maize, we established the Trojan Horse approach. Here, tagged peptides are delivered into the maize apoplast in a highly localized fashion by using a genetically modified version of the biotrophic pathogen Ustilago maydis. This technique offers the possibility of rapid testing of predicted maize peptides for in situ functions.