Quantitative Measurement of Pattern-Triggered ROS Burst as an Early Immune Response in Tomato

The rapid accumulation of extracellular "reactive oxygen species" (ROS), also known as the "oxidative burst", is an early plant immune response triggered by pathogen-derived microbe-associated molecular patterns and by endogenous plant signaling molecules. The oxidative burst is often used as a readout for the activation of defense signaling. Here, we present a detailed protocol for the continuous measurement of ROS production in leaf discs of tomato plants, using a chemiluminescence-based assay in a microtiter plate format. We also include recommendations for data analysis and for the quantitative assessment of differences in ROS burst dynamics, as caused by different types of elicitors, or in different tomato genotypes.