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The auditory function in migraine model rats induced by postauricular nitroglycerin injection.
Rongxiang Qi1, Jilei Zhang1, Tongxiang Diao1
1Department of Otolaryngology, Head and Neck Surgery, People's Hospital, Peking University, Beijing, China.
Frontiers in Neurology
|November 29, 2023
Summary
This study introduces a novel rat model for migraines, demonstrating that postauricular nitroglycerin (NTG) injection effectively induces migraine and hearing loss. This model offers a safer alternative for studying the link between migraines and auditory dysfunction.
Area of Science:
- Neuroscience
- Otolaryngology
- Pharmacology
Background:
- Migraine-associated inner ear symptoms lack clear mechanistic understanding.
- Previous animal models showed altered auditory brainstem response (ABR) latency but not threshold.
- A need exists for a migraine model incorporating hearing loss to study auditory function impacts.
Purpose of the Study:
- To establish a rat model of migraine with associated hearing loss.
- To compare the efficacy and safety of postauricular versus intraperitoneal nitroglycerin (NTG) injection for migraine induction.
- To investigate the impact of NTG-induced migraine on auditory function.
Main Methods:
- Migraine model induced via postauricular or intraperitoneal NTG injection in rats.
- Repeated drug administration over 5 alternate days with body weight monitoring.
- Assessment of tactile hyperalgesia using von-Frey filaments and auditory function via ABR.
Main Results:
- Postauricular NTG injection induced hyperalgesia without affecting weight gain.
- Intraperitoneal NTG injection resulted in slowed or negative weight gain.
- Postauricular NTG increased ABR thresholds and prolonged latency at 4 and 8 kHz, with greater impact on the right ear.
Conclusions:
- Postauricular NTG injection is a safer and more effective method for creating a rat migraine model compared to intraperitoneal injection.
- This model demonstrates significant damage to auditory function, suggesting a link to cochlear issues.
- The postauricular NTG-induced migraine rat model may serve as a valuable tool for studying cochlear migraines.
