Related Experiment Video
Updated: Jul 9, 2025
Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
Published on: March 18, 2014
Nelumbo nucifera Leaf Extract Induces Cytotoxicity in Osteosarcoma Saos-2 Cells
Gautam Britina1, Devaraj Ezhilarasan2, Karthik Shree Harini2
1Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Abstract:
Background Osteosarcoma is the eighth most common cancer and its prevalence in children makes it a global concern. Existing medications and treatments like high-dose methotrexate possess harmful side effects. Therefore, novel herbal drugs like Nelumbo nucifera are of utmost importance. Aim To analyze a novel anticancer herbal drug, Nelumbo nucifera leaf extract for its cytotoxic potential against osteosarcoma. Materials and method Nelumbo nucifera leaf extract was prepared. Saos-2 Cells (human osteosarcoma cell line) were treated with Nelumbo nucifera leaf extract (25, 50, 75, 100, 125, and 150 µg/ml) for 24 hours which were then subjected to MTT assay, morphological analysis and DAPI staining. Results The results suggested that Nelumbo nucifera leaf extract had a concentration-dependent cytotoxic effect on Saos-2 cell line. The extract significantly reduced the number of viable cells, inhibited proliferation and induced morphological changes in Saos-2 cells. Conclusion Nelumbo nucifera has the potential to induce cytotoxicity against osteosarcoma cell lines and hence, this study provides a novel therapeutic regimen for the treatment of osteosarcoma.
Insights
Nelumbo nucifera leaf extract shows promise as a natural treatment for osteosarcoma. This herbal remedy demonstrated significant cancer cell death in laboratory tests, offering a potential alternative to conventional therapies.
Area of Science:
- Phytochemistry
- Oncology
- Pharmacology
Background:
- Osteosarcoma, a prevalent childhood cancer, necessitates safer treatment options due to severe side effects of current therapies like methotrexate.
- The exploration of natural compounds, such as Nelumbo nucifera (sacred lotus), is crucial for developing novel anticancer drugs.
Purpose of the Study:
- To investigate the cytotoxic potential of Nelumbo nucifera leaf extract against human osteosarcoma cells.
- To evaluate Nelumbo nucifera as a potential novel therapeutic agent for osteosarcoma.
Main Methods:
- Nelumbo nucifera leaf extract was prepared and applied to Saos-2 osteosarcoma cells at varying concentrations (25-150 µg/ml) for 24 hours.
- Cytotoxicity was assessed using MTT assay, morphological analysis, and DAPI staining.
Main Results:
- Nelumbo nucifera leaf extract exhibited a dose-dependent cytotoxic effect on Saos-2 cells.
- The extract significantly reduced cell viability, inhibited proliferation, and induced characteristic morphological changes indicative of cell death.
Conclusions:
- Nelumbo nucifera demonstrates significant potential to induce cytotoxicity in osteosarcoma cell lines.
- This study suggests Nelumbo nucifera as a promising candidate for a novel therapeutic strategy against osteosarcoma.
More Related Videos
08:52Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
Published on: June 13, 2018
08:47Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
Published on: August 21, 2012