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Molecular Characterization and Antibiotic Susceptibility Pattern of Bacterial Strains Isolated From Wound of Patients

Eemaz Nathaniel1, Jibran Ikram2, Aimen James3

  • 1Research, Rehman Medical Institute, Peshawar, PAK.

Cureus
|November 29, 2023
PubMed
Abstract

Insights

Diabetic wound infections show increasing antibiotic resistance. Gram-positive bacteria resist penicillin, while gram-negative bacteria resist amoxicillin and trimethoprim/sulfamethoxazole.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Diabetic patients are immunocompromised, increasing susceptibility to wound infections.
  • Staphylococcus aureus is a common pathogen in diabetic wounds.
  • Comprehensive analysis of antibiotic resistance in diabetic wound pathogens is crucial.

Purpose of the Study:

  • To investigate the prevalence of multiple antibiotic resistance in bacterial pathogens from diabetic wound infections.
  • To assess the susceptibility patterns of bacterial isolates to commonly used antibiotics.

Main Methods:

  • Cross-sectional study conducted at a tertiary care facility.
  • Aseptic collection of 140 samples from diabetic patients' wound lesions.
  • Antibiotic susceptibility testing of 122 bacterial isolates using various media and cultures.

Main Results:

  • Gram-positive isolates showed high resistance to penicillin G (93.18%) but sensitivity to vancomycin (100%) and linezolid (95%).
  • Gram-negative isolates exhibited 100% resistance to amoxicillin and sulfamethoxazole/trimethoprim.
  • Significant levels of antibiotic resistance were observed in bacterial pathogens from diabetic wounds.

Conclusions:

  • A notable increase in antibiotic resistance among diabetic wound pathogens has been observed.
  • Urgent need for updated treatment guidelines and development of new therapeutic strategies.
Keywords:
antibiotic susceptibilitybacterial strainscross-sectional studydiabetic patientsmolecular characterizationwound infections

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