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Comprehensive bioinformatics analysis revealed potential key genes and pathways underlying abdominal aortic aneurysm
Kaijie Zhang1, Jianing Yue2, Li Yin1
1Department of Vascular Surgery, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province 310009, China.
Computational and Structural Biotechnology Journal
|November 29, 2023
Summary
Researchers identified key genes and pathways involved in abdominal aortic aneurysm (AAA) using RNA-sequencing. This discovery may lead to new drug therapies for this life-threatening condition.
Area of Science:
- Vascular Biology
- Genomics
- Bioinformatics
Background:
- Abdominal aortic aneurysm (AAA) is a dangerous aortic dilatation with high rupture mortality.
- Identifying key genes and pathways is crucial for developing effective AAA treatments.
Purpose of the Study:
- To comprehensively identify potential key genes and pathways in AAA pathogenesis.
- To validate candidate genes and explore potential therapeutic targets.
Main Methods:
- RNA sequencing (RNA-seq) of abdominal aortic tissues from AAA patients and controls.
- Integrated bioinformatic analysis, including Gene Ontology (GO), KEGG, and Protein-Protein Interaction (PPI) network analysis.
- Validation using Gene Expression Omnibus (GEO) datasets, immunohistochemistry, and single-cell RNA sequencing.
Main Results:
- 1148 differentially expressed genes (DEGs) were identified, with enrichment in extracellular matrix organization and ECM-receptor interaction pathways.
- Six key genes (CXCL8, CCL2, PTGS2, SELL, CCR7, CXCL1) were validated and showed high discriminatory ability between AAA and normal tissues.
- Differential gene distribution across cell types and 26 potential drugs linked to these key genes were identified.
Conclusions:
- This study provides a comprehensive evaluation of key genes and pathways implicated in AAA.
- The identified genes and potential drugs offer promising avenues for developing curative pharmacological therapies for AAA.
