Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

4.6K
4.6K
Mismatch Repair01:20

Mismatch Repair

4.9K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.9K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K
Microtubule Instability02:17

Microtubule Instability

5.1K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.1K
Crossing Over01:30

Crossing Over

4.4K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.4K
RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Screening metatranscriptomes for ultrastable RNA secondary structures reveals hidden bacteriophages and novel capsid nanomaterials.

bioRxiv : the preprint server for biology·2026
Same author

Detecting chronically infecting viruses of haloarchaea.

FEMS microbiology letters·2026
Same author

CRISPR-Cas targeting in <i>Haloferax volcanii</i> promotes within-species gene exchange by triggering homologous recombination.

microLife·2026
Same author

A previously undescribed archaeal virus suppresses host immunity.

EMBO reports·2025
Same author

Evolutionary insights into provirus-encoded CRISPR-Cas systems in halophilic archaea.

microLife·2025
Same author

AI-directed gene fusing prolongs the evolutionary half-life of synthetic gene circuits.

Science advances·2025

Related Experiment Video

Updated: Jul 9, 2025

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

14.7K

Neighboring inteins interfere with one another's homing capacity.

Israela Turgeman-Grott1, Danielle Arsenault2, Dekel Yahav1

  • 1The Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, P.O. Box 39040, 6997801 Tel Aviv, Israel.

PNAS Nexus
|November 29, 2023
PubMed
Summary

Inteins are mobile genetic elements. In Haloarchaea, the minichromosome maintenance (MCM) protein

Keywords:
co-hominghaloarchaeahoming endonucleaseintein

More Related Videos

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

409.0K
Assembly and Purification of Prototype Foamy Virus Intasomes
10:20

Assembly and Purification of Prototype Foamy Virus Intasomes

Published on: March 19, 2018

5.5K

Related Experiment Videos

Last Updated: Jul 9, 2025

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

14.7K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

409.0K
Assembly and Purification of Prototype Foamy Virus Intasomes
10:20

Assembly and Purification of Prototype Foamy Virus Intasomes

Published on: March 19, 2018

5.5K

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Inteins are mobile genetic elements found across life, capable of invading host genes.
  • The minichromosome maintenance (MCM) protein, crucial for DNA replication, presents multiple intein insertion sites in Haloarchaea.
  • Specific intein insertion sites (MCM-a and MCM-d) are frequently targeted within the MCM protein.

Purpose of the Study:

  • To investigate intein invasion dynamics and interactions between adjacent inteins in the MCM protein of *Haloferax mediterranei*.
  • To analyze the frequency and conservation of intein insertion sites in MCM homologs across the Haloferacales order.
  • To understand the mechanisms governing intein homing and splicing in haloarchaeal MCM proteins.

Main Methods:

  • In vivo studies in *Haloferax mediterranei* to observe intein invasion and splicing.
  • Computational analysis of MCM homologs from 129 Haloferacales species.
  • Assessing intein recognition, cleavage, and homing efficiency under different invasion scenarios.

Main Results:

  • Inteins at MCM-a and MCM-d sites are recognized and cleave their targets.
  • Single intein homing is efficient, but co-homing into intein-free sites is less effective.
  • Invasion is more successful when one intein is already present at a neighboring site.
  • A specific amino acid substitution (Proline for Serine) at the MCM-d site prevents splicing but not recognition.

Conclusions:

  • Intein invasion and homing in haloarchaeal MCM proteins exhibit complex dynamics, influenced by the presence of neighboring inteins.
  • The efficiency of intein homing is context-dependent, favoring invasion of already partially invaded alleles.
  • Specific extein residues can modulate intein splicing without abolishing intein recognition, highlighting intricate host-intein interactions.