Related Experiment Video

Updated: Aug 14, 2026

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Methylmalonic aciduria and propionic acidaemia studied by proton nuclear magnetic resonance spectroscopy

R A Iles, R A Chalmers, A J Hind

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |December 15, 1986
    PubMed

    More Related Videos

    Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging11:43

    Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

    Published on: December 30, 2016

    Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy07:02

    Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

    Published on: December 16, 2021

    Related Concept Videos

    Proton (¹H) NMR: Chemical Shift01:07

    Proton (¹H) NMR: Chemical Shift

    Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
    Absorption signals of all the protium nuclei in a...
    ¹H NMR: Complex Splitting01:13

    ¹H NMR: Complex Splitting

    A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
    Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
    ¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

    ¹H NMR of Labile Protons: Deuterium (²H) Substitution

    This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

    When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
    NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones01:15

    NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones

    In aldehydes, the hydrogen atom connected to the carbonyl carbon helps distinguish aldehydes from other carbonyl compounds using ¹H NMR spectroscopy. The closeness of aldehydic hydrogen to the electrophilic carbonyl carbon highly deshields the hydrogen atom causing its signal to appear around 10 ppm in the ¹H NMR spectra. α hydrogens split the aldehydic proton signal, which helps identify the number of α hydrogens in the molecule. For instance, one α hydrogen creates a doublet for an aldehydic...
    NMR and Mass Spectroscopy of Carboxylic Acids01:30

    NMR and Mass Spectroscopy of Carboxylic Acids

    In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
    While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
    Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the disappearance of the acidic...