Extended application of RP-HPLC for characterization of human hemoglobin variants

The different steps for the structural study of a human hemoglobin, from protein isolation to amino acid analysis, can be achieved using as the only method reversed phase high performance liquid chromatography. HPLC allows effective and rapid separations on microquantities of material. The miniaturization of the techniques led to a new approach involving successive enzymic hydrolysis of peptides.