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Targeted down-regulation of SRSF1 exerts anti-cancer activity in OSCC through impairing lysosomal function and

Yi Qu1, Ying He1, Yijuan Wang2

  • 1Department of Oral and Maxillofacial & Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China.

Iscience
|November 29, 2023
PubMed

Insights

Targeting serine/arginine-rich splicing factor 1 (SRSF1) inhibits oral squamous cell carcinoma (OSCC) growth. This study reveals SRSF1 knockdown impedes tumor progression by disrupting lysosomal biogenesis and autophagy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent head and neck cancer with limited targeted therapies.
  • Serine/arginine-rich splicing factor 1 (SRSF1), a proto-oncogene, is implicated in tumor suppression, but its role in OSCC is unclear.

Purpose of the Study:

  • To investigate the expression, function, and mechanism of SRSF1 in OSCC.
  • To determine the prognostic significance of SRSF1 in OSCC patients.
  • To evaluate SRSF1 as a potential therapeutic target for OSCC.

Main Methods:

  • Retrospective analysis of clinical OSCC samples.
  • In vitro experiments involving SRSF1 knockdown in OSCC cell lines.
  • Construction of cell rescue models to assess lysosomal and autophagy pathways.
  • In vivo validation using xenograft models.

Main Results:

  • Elevated SRSF1 expression correlates with poor prognosis in OSCC patients.
  • SRSF1 knockdown significantly inhibits OSCC cell growth, survival, lysosomal biogenesis, and autophagy.
  • Targeted SRSF1 inhibition suppresses OSCC growth in xenograft models by affecting these pathways.

Conclusions:

  • SRSF1 plays a critical role in OSCC progression.
  • Targeting SRSF1 represents a promising therapeutic strategy for OSCC.
  • Inhibition of SRSF1 suppresses OSCC by disrupting lysosomal biogenesis and autophagy.
Keywords:
CancerDrugsMolecular biology

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