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Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure.

Yuanmeng Qi1, Huifang Si1,2, Xiaofei Jin3

  • 1Department of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, China.

Frontiers in Public Health
|November 29, 2023
PubMed
Summary

Manganese (Mn) exposure in workers elevates TIM-3 and lowers complement C3 levels, indicating potential immune system damage. These markers may serve as biomarkers for Mn occupational hazards.

Keywords:
Mn-exposed workersRBC MnTIM-3complement C3immunotoxicity

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Area of Science:

  • Occupational Health
  • Immunotoxicology
  • Environmental Medicine

Background:

  • Manganese (Mn) is vital for biological functions but excessive exposure causes immune damage.
  • Occupational Mn exposure is a significant hazard, yet its immunotoxic effects on workers and reliable biomarkers remain under-researched.
  • Current understanding of Mn's impact on the occupational immune system is incomplete.

Purpose of the Study:

  • To investigate the immunotoxicity of Manganese (Mn) exposure in occupationally exposed workers.
  • To evaluate Mn's effect on the soluble immune checkpoint TIM-3 (T-cell immunoglobulin and mucin containing protein 3) and complement C3.
  • To identify potential biomarkers for Mn exposure in the occupational population.

Main Methods:

  • Recruited 144 Mn-exposed workers from manufacturing and railroad industries.
  • Measured Red blood cell Manganese (RBC Mn) concentration using inductively coupled plasma mass spectrometry.
  • Assessed serum complement C3 and TIM-3 levels via ELISA kits and analyzed dose-response relationships.

Main Results:

  • Mn exposure was associated with significantly elevated serum TIM-3 expression and decreased serum complement C3 expression.
  • A clear dose-response relationship was observed between RBC Mn concentration and serum TIM-3 and complement C3 levels.
  • Complement C3 was found to significantly mediate the relationship between RBC Mn and TIM-3 expression.

Conclusions:

  • Manganese exposure negatively impacts the immune system in occupational settings, evidenced by altered TIM-3 and C3 levels.
  • Serum TIM-3 and complement C3 show promise as potential biomarkers for assessing Mn exposure and its immunotoxicity.
  • Findings offer insights for preventing and managing Mn-related occupational health risks.