Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Opening the black box: in situ imaging of arbuscular mycorrhizal fungal structures in soil using synchrotron-based micro-CT.

The New phytologist·2026
Same author

Ovalbumin oxidative modification fingerprints depend on gas plasma-driven reactive species profiles.

Redox report : communications in free radical research·2026
Same author

Oxidative modifications of tuberculosis antigen Ag85B alter its T cell antigenicity.

Redox biology·2026
Same author

Distinct Extracellular Matrix Protein Signatures of Cortical and Cancellous Bone Allografts Following Processing for Clinical Use.

Cells·2026
Same author

Improved in vivo bone regeneration and mechanical stability in critical-sized defects using WZM211 fluorine coated fibres.

Acta biomaterialia·2026
Same author

Redox processes in the treatment of advanced skin cancers.

Clinical & experimental metastasis·2026
Same journal

Development of a fast-crosslinking hydrogel system doped with magnetic mesoporous nanoparticles for sustained fluoride ion release and caries prevention.

Frontiers in bioengineering and biotechnology·2026
Same journal

Editorial: Advancements in research on plant-derived extracellular vesicles and nanoparticles- applications in biotechnology and one health.

Frontiers in bioengineering and biotechnology·2026
Same journal

Operational integrity screening for telemedicine workflows: an explainable motion and audiovisual coherence framework.

Frontiers in bioengineering and biotechnology·2026
Same journal

Advances in biomechanical modeling of lumbar spine diseases and tumors: gaps, opportunities, and AI integration.

Frontiers in bioengineering and biotechnology·2026
Same journal

Engineering <i>Lactococcus cremoris</i> strains co-expressing two cellulase genes for growth on cellulose.

Frontiers in bioengineering and biotechnology·2026
Same journal

Exosome-mediated tendon-derived stem cell therapy strategies: potential and challenges.

Frontiers in bioengineering and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jul 9, 2025

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

11.5K

Cold physical plasma treatment optimization for improved bone allograft processing.

Maximilian Fischer1, Emely Bortel2, Janosch Schoon1

  • 1Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany.

Frontiers in Bioengineering and Biotechnology
|November 29, 2023
PubMed
Summary
This summary is machine-generated.

Cold physical plasma (CPP) treatment of bone allografts shows potential for improving cell viability and function without altering bone morphology. Optimized gas mixtures and treatment times are key for enhanced bone regeneration.

Keywords:
allograftscancellous bonecold atmospheric pressure plasmamesenchymal stromal cellsplasma medicinesynchrotron radiation computed tomography

More Related Videos

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

12.9K
Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
06:36

Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet

Published on: November 2, 2020

4.1K

Related Experiment Videos

Last Updated: Jul 9, 2025

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

11.5K
Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells
07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

Published on: November 17, 2017

12.9K
Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
06:36

Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet

Published on: November 2, 2020

4.1K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Surgery

Background:

  • Large bone defects pose challenges in musculoskeletal surgery, often requiring bone graft substitutes.
  • Current bone allograft processing methods can limit host bone ingrowth.
  • Cold physical plasma (CPP) offers potential for modifying implant surfaces to enhance cellular integration.

Purpose of the Study:

  • To pre-clinically assess the feasibility of processing allogeneic bone grafts with CPP.
  • To analyze bone morphology changes using synchrotron radiation-based microcomputed tomography (SR-µCT).
  • To evaluate the impact of CPP on human bone cell viability and function.

Main Methods:

  • Allogeneic bone grafts were processed using CPP with varying gas mixtures and durations.
  • Synchrotron radiation-based microcomputed tomography (SR-µCT) was used for detailed morphological analysis.
  • In vitro assays assessed human bone cell viability and alkaline phosphatase activity.

Main Results:

  • CPP treatment did not significantly alter key bone morphological properties (volume, surface area, porosity).
  • Cellular metabolic activity and alkaline phosphatase activity showed varied responses to different CPP parameters.
  • A 3-minute CPP treatment with a He + 0.1% N2 gas mixture significantly enhanced bone cell viability and alkaline phosphatase activity.

Conclusions:

  • Pre-therapeutic CPP processing of bone allografts is a promising approach for enhancing regenerative potential.
  • Optimization of gas composition and treatment duration is crucial for maximizing beneficial effects.
  • Further research is needed to refine CPP parameters for specific clinical applications in bone regeneration.