Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinico-Pathologic Features of Adult Erdheim-Chester Disease in South Korea: A Multicenter Retrospective Study (KCSG LY22-15).

Cancer research and treatment·2026
Same author

Kidney Hematopoietic Stem and Progenitor Cells Contribute to Myeloid Development and Pathology in Lupus Nephritis.

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

Pellino1-mTOR/S6K1 signaling axis is a key pathogenesis for the development of polycystic kidney disease.

Cell death & disease·2026
Same author

Spatial immunophenotyping of tumor-associated macrophages predicts prognostic outcomes in clear cell renal cell carcinoma.

Urologic oncology·2026
Same author

Impact of estrogen receptor expression levels on chemo-responsiveness and prognosis of breast cancer patients treated with neoadjuvant chemotherapy.

NPJ breast cancer·2026
Same author

Multiplex Immunofluorescence Assay with Opal Reagents for Identifying Mononuclear Cell Subsets in Kidney Allograft Rejection Types.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jul 9, 2025

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.4K

Solution-free and simplified H&E staining using a hydrogel-based stamping technology.

Jinho Kim1, Woongsun Choi1, Dahyeon Yoo1

  • 1Noul Co., Ltd., Yongin-si, Republic of Korea.

Frontiers in Bioengineering and Biotechnology
|November 29, 2023
PubMed
Summary
This summary is machine-generated.

This study presents a novel solution-free hematoxylin and eosin (H&E) staining method using agarose hydrogel patches. This innovative technique simplifies the staining process, reduces errors, and ensures consistent results for disease diagnosis.

Keywords:
agarosecancereosinhematoxylinhistopathologyhydrogelsolution-free stainingtissue staining

More Related Videos

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
12:38

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Published on: December 16, 2011

14.8K
Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

18.8K

Related Experiment Videos

Last Updated: Jul 9, 2025

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.4K
Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
12:38

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Published on: December 16, 2011

14.8K
Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

18.8K

Area of Science:

  • Histopathology
  • Biomedical Engineering
  • Materials Science

Background:

  • Hematoxylin and eosin (H&E) staining is crucial for disease diagnosis and biological research.
  • Conventional H&E staining is solution-based, complex, and prone to errors and artifacts, impacting diagnostic accuracy.
  • Inconsistent staining results challenge reliable interpretation in medical diagnostics.

Purpose of the Study:

  • To develop a solution-free H&E staining method to overcome limitations of conventional techniques.
  • To improve consistency and reduce user-handling errors in H&E staining.
  • To create an accessible H&E staining method for resource-limited settings.

Main Methods:

  • A novel solution-free H&E staining method utilizing agarose hydrogel patches.
  • Serial stamping technique with hydrogel patches containing hematoxylin and eosin dyes.
  • Adjustable staining intensity by controlling stamping time.

Main Results:

  • Minimized color variation and handling errors through the stamping process.
  • Effective control over staining intensity, addressing artifacts from tissue processing and thickness.
  • Achieved staining quality equivalent to conventional methods without water or solutions.

Conclusions:

  • The hydrogel-based H&E staining method offers a simplified, consistent, and artifact-resistant alternative.
  • This solution-free approach is suitable for resource-limited environments, enhancing diagnostic capabilities.
  • The method provides a valuable tool for researchers and medical professionals in diverse settings.