Related Experiment Video
Updated: Jul 9, 2025
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
Published on: March 5, 2022
Integrative transcriptome analysis reveals alternative polyadenylation potentially contributes to GCRV early
Sheng Tan1, Jie Zhang2,3, Yonglin Peng1
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai, Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.
Introduction:
Grass carp reovirus (GCRV), a member of the Aquareovirus genus in the Reoviridae family, is considered to be the most pathogenic aquareovirus. Productive viral infection requires extensive interactions between viruses and host cells. However, the molecular mechanisms underlying GCRV early infection remains elusive.
Methods:
In this study we performed transcriptome and DNA methylome analyses with Ctenopharyngodon idellus kidney (CIK) cells infected with GCRV at 0, 4, and 8 h post infection (hpi), respectively.
Results:
We found that at early infection stage the differentially expressed genes related to defense response and immune response in CIK cells are activated. Although DNA methylation pattern of CIK cells 8 hpi is similar to mock-infected cells, we identified a considerable number of genes that selectively utilize alternative polyadenylation sites. Particularly, we found that biological processes of cytoskeleton organization and regulation of microtubule polymerization are statistically enriched in the genes with altered 3'UTRs.
Discussion:
Our results suggest that alternative polyadenylation potentially contributes to GCRV early infection.
Related Concept Videos
02:28Leaky Scanning
02:24Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...