Effects of paclitaxel before vitrification on the nuclear maturation and development of immature porcine oocytes
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Updated: Jul 9, 2025
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
M Tone1, R Ukyo2, S H Sakamoto2
1Animal Reproduction Laboratory, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Background:
Cryopreservation of porcine oocytes is difficult compared with other species and immature oocytes particularly so compared to the meiotic stage.
Objective:
To evaluate the efficacy of a pretreatment with 1 micromole per L paclitaxel (PTX, 30 min exposure) before vitrification to promote the maturation of porcine immature oocytes.
Materials And Methods:
Cumulus cell-enclosed oocytes (COs) aspirated from porcine ovaries were divided into three groups: i) non-pretreated with PTX and non-vitrified group (control group); ii) pretreated with PTX and vitrified group (PTX-V group); and iii) non-pretreated with PTX and vitrified group (nPTX-V group).
Results:
The nuclear maturation rate up to the preovulatory stage was significantly lower (P < 0.05) in the nPTX-V group than in the control group, but was similar in the PTX-V and control groups. No significant differences were observed in viability assessed by a normal CO morphology and the embryonic development of oocytes activated by the parthenogenetic stimulation between the PTX-V and control groups, but not the non-PTX-V group.
Conclusion:
PTX may promote the maturation of vitrified porcine immature oocytes. Doi.org/10.54680/fr23510110812.
