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Updated: Jul 9, 2025
Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model
Published on: June 18, 2013
Recommendations from the COST action CA17116 (SPRINT) for the standardization of perinatal derivative preparation and
Aleksandar Janev1, Asmita Banerjee2,3, Adelheid Weidinger2,3
1Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Insights
Perinatal derivatives (PnD) show therapeutic potential, but inconsistent research methods hinder clinical use. This study introduces an e-questionnaire and consensus criteria to standardize PnD research and accelerate translation.
Area of Science:
- Biomedical research
- Regenerative medicine
- Cell therapy
Background:
- Perinatal derivatives (PnD) possess therapeutic properties for various diseases.
- Lack of standardized methods in PnD research delays clinical translation.
- Current PnD research lacks critical evaluation of preparation and in vitro testing protocols.
Purpose of the Study:
- To assess the current state of PnD research methods.
- To propose consensus criteria for PnD standardization.
- To facilitate the clinical translation of PnD research.
Main Methods:
- Development of the PnD e-questionnaire.
- Analysis of published literature on PnD procurement, isolation, culturing, preservation, and characterization.
- Proposal of minimal reporting criteria for PnD research.
Main Results:
- The PnD e-questionnaire assesses current methodologies in PnD research.
- Identified a need for standardized reporting of PnD characterization.
- Established consensus criteria for PnD research transparency and reproducibility.
Conclusions:
- Standardization of PnD research is crucial for clinical translation.
- The PnD e-questionnaire and proposed criteria will guide researchers.
- This initiative aims to accelerate the clinical application of PnD therapies.
Abstract:
Many preclinical studies have shown that birth-associated tissues, cells and their secreted factors, otherwise known as perinatal derivatives (PnD), possess various biological properties that make them suitable therapeutic candidates for the treatment of numerous pathological conditions. Nevertheless, in the field of PnD research, there is a lack of critical evaluation of the PnD standardization process: from preparation to in vitro testing, an issue that may ultimately delay clinical translation. In this paper, we present the PnD e-questionnaire developed to assess the current state of the art of methods used in the published literature for the procurement, isolation, culturing preservation and characterization of PnD in vitro. Furthermore, we also propose a consensus for the scientific community on the minimal criteria that should be reported to facilitate standardization, reproducibility and transparency of data in PnD research. Lastly, based on the data from the PnD e-questionnaire, we recommend to provide adequate information on the characterization of the PnD. The PnD e-questionnaire is now freely available to the scientific community in order to guide researchers on the minimal criteria that should be clearly reported in their manuscripts. This review is a collaborative effort from the COST SPRINT action (CA17116), which aims to guide future research to facilitate the translation of basic research findings on PnD into clinical practice.
