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Related Experiment Videos

Corneal epithelial Cl-dependent pump quantified.

W H Beekhuis, B E McCarey

    Experimental Eye Research
    |November 1, 1986
    PubMed
    Summary
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    Rabbit corneas thin when covered with silicone oil, indicating an active epithelial pump mechanism. This epithelial pump rate is crucial for accurate in vitro measurements of corneal endothelial function.

    Area of Science:

    • Ophthalmology
    • Corneal Physiology
    • Cellular Transport

    Background:

    • The corneal endothelium actively pumps fluid to maintain corneal transparency.
    • Accurate measurement of endothelial pump function in vitro requires understanding all contributing fluid transport mechanisms.
    • The role of the corneal epithelium in fluid transport under experimental conditions is not fully elucidated.

    Purpose of the Study:

    • To quantify the fluid transport rate of the corneal epithelium in an in vitro rabbit cornea model.
    • To determine the contribution of the epithelial pump to overall corneal thickness changes.
    • To establish the necessity of accounting for epithelial transport in endothelial function studies.

    Main Methods:

    • Rabbit corneas were perfused, and thickness changes were monitored.

    Related Experiment Videos

  • Both epithelial and endothelial surfaces were covered with silicone oil to isolate transport mechanisms.
  • Experiments involved endothelial removal, epithelial abrasion, temperature changes, and perfusion with chloride-free solutions.

    • Main Results:

    • A constant corneal thinning rate of 11.1 microns/hr was observed with silicone oil application.
    • Epithelial abrasion and cooling to 0°C significantly reduced or abolished thinning.
    • Perfusion with a chloride-free solution also prevented significant thinning, suggesting an active epithelial transport process.
    • The calculated epithelial pump rate was approximately 1.2 microns L hr⁻¹ cm⁻².

    Conclusions:

    • The corneal epithelium possesses an active fluid pump mechanism.
    • This epithelial pump contributes significantly to fluid transport and must be considered in in vitro studies.
    • Accurate assessment of corneal endothelial function requires accounting for the quantified epithelial pump rate.