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Published on: December 5, 2020
Thirteen new peptaibols with antimicrobial activities from Trichoderma sp
Pan Tang1, Dan Huang1, Kai-Xuan Zheng1
1Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou 510632, China.
Researchers discovered novel peptaibols from Trichoderma fungi, including unique 14-residue compounds. These peptaibols show moderate antimicrobial activity against bacteria like Staphylococcus aureus and fungi such as Candida albicans.
Area of Science:
- Natural Product Chemistry
- Mycology
- Medicinal Chemistry
Background:
- Peptaibols are peptide antibiotics produced by fungi.
- Trichoderma species are known sources of bioactive natural products.
- Exploring fungal metabolites is crucial for discovering new antimicrobial agents.
Purpose of the Study:
- To isolate and characterize novel peptaibols from Trichoderma sp.
- To determine the structures and absolute configurations of new peptaibols.
- To evaluate the antimicrobial activities of the isolated peptaibols.
Main Methods:
- Isolation of peptaibols using chromatographic techniques.
- Structure elucidation via Nuclear Magnetic Resonance (NMR) spectroscopy and High-Resolution Electrospray Ionization Tandem Mass Spectrometry (HR-ESI-MS/MS).
- Determination of absolute configurations using Marfey's method.
Main Results:
- Seven novel 18-residue peptaibols (neoatroviridins E-K) and six new 14-residue peptaibols (harzianins NPDG J-O) were identified.
- Compounds 12 and 13 are the first reported 14-residue peptaibols containing an acidic amino acid residue.
- All 18-residue peptaibols displayed moderate activity against Staphylococcus aureus (MIC 8-32 μg·mL⁻¹).
- Compound 9 showed moderate activity against Candida albicans (MIC 16 μg·mL⁻¹).
Conclusions:
- The study expands the known diversity of peptaibols from Trichoderma sp.
- Novel peptaibols with unique structural features were discovered.
- The isolated peptaibols exhibit potential as antimicrobial agents against specific bacterial and fungal pathogens.
