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Use of in vivo kinetic techniques to evaluate metabolism of nutrients
Abstract:
Most nutrition research is related to rates of physiological processes. Information about those processes can be gained by in vivo kinetic techniques; however, many nutritionists are hesitant to use in vivo kinetics. The two basic in vivo kinetic techniques are single injection and continuous infusion of tracer into a pool of tracee. Either technique can form the basis for multiple-pool kinetics, or modeling. Solving a multiple-pool system can provide flow rates of substances between metabolic pools and is valuable for understanding a particular metabolic pathway or process. In vivo kinetic techniques can be valuable in understanding mechanisms whereby partitioning agents affect the distribution of nutrients, especially protein and fat, in food-producing animals. In vivo kinetics is a valuable tool for nutrition research and should be used more frequently.