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Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery

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Kidney Immune Cell Characterization of Humanized Mouse Models.

Sanjeev Noel¹, Johanna T Kurzhagen¹, Sul A Lee¹

¹Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

|December 1, 2023

Summary

This summary is machine-generated.

Humanized mouse models show promise for studying kidney diseases. Certain models, like huNOG and huNOGEXL, better reflect human kidney immune cell composition for translational research.

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Area of Science:

Immunology
Translational Medicine
Renal Research

Background:

Experimental studies often lack clinical translation due to biological differences.
Humanized mouse models aim to bridge the gap between preclinical research and human disease.
Data on humanized models for renal immune cell-mediated diseases are scarce.

Purpose of the Study:

To investigate the immune cell composition in the kidneys of different humanized mouse models.
To compare these models with normal human kidney immune cell profiles.
To assess the suitability of these models for translational studies in renal diseases.

Main Methods:

Immunophenotyping of kidneys from NOG (EXL) and NSG mouse strains engrafted with human CD34+ hematopoietic stem cells or PBMCs.
Comparison of immune cell composition in kidney, blood, spleen, and bone marrow.
Flow cytometry analysis to confirm engraftment and quantify immune cell populations.

Main Results:

Significant engraftment of human immune cells observed in blood and kidneys across all tested models.
huNOG and huNOGEXL models demonstrated immune cell compositions more representative of normal human kidneys compared to huNSG.
Variability in immune cell composition was noted among the different humanized mouse models.

Conclusions:

Humanization leads to immune cell infiltration in mouse kidneys, with varying compositions depending on the model.
huNOG and huNOGEXL models show potential for translational studies in human renal diseases.
Further validation is needed to optimize humanized models for specific renal research applications.