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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
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Fluorescent-Probe Characterization for Pore-Space Mapping with Single-Particle Tracking.

Rafael Mayorga González1, J J Erik Maris1, Marita Wagner1

  • 1Inorganic Chemistry and Catalysis, Debye Institute for Nanomaterials Science and Institute for Sustainable and Circular Chemistry, Utrecht University, Universiteitsweg 99, 3584, CG Utrecht, The Netherlands.

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|December 1, 2023
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Summary

Researchers used quantum dot probes in model pores to map complex pore networks. By controlling probe-host interactions, they accurately characterized pore accessibility in various materials.

Keywords:
DiffusionNanofluidicsPorosity CharacterizationSingle Particle TrackingSingle-Molecule Localization Microscopy

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Physical Chemistry

Background:

  • Porous solids feature intricate pore networks crucial for material properties.
  • Characterizing these networks at the nanoscale requires understanding fluorescent probe diffusion.
  • Confinement effects on probe motion are vital for accurate pore network analysis.

Purpose of the Study:

  • To develop model systems for studying fluorescent probe behavior in confined environments.
  • To investigate the impact of probe-host interactions on diffusion and trapping.
  • To establish experimental conditions for mapping accessible pore space using single-emitter probes.

Main Methods:

  • Fabrication of well-defined lithography-made model pores.
  • Utilizing pH-responsive quantum dot probes to control surface interactions.
  • Single-particle tracking of quantum dots within model and real-world porous structures.

Main Results:

  • Demonstrated suppression of probe trapping at pore walls by tuning pH-responsiveness.
  • Successfully mapped the accessible pore space of a 1D pore array.
  • Validated the method for characterizing a real-life polymerization-catalyst-support particle.

Conclusions:

  • Controlling probe-host interactions is key to reliable pore network characterization.
  • Lithography-made model pores provide a robust platform for studying confinement effects.
  • This technique offers a high-resolution method for analyzing complex porous materials.