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pH biosensors based on hydrogel optical fiber.

Zhenglan Bian, Caihong Dai, Fenghong Chu

    Applied Optics
    |December 1, 2023
    PubMed
    Summary

    This study introduces a novel hydrogel optical fiber pH sensor using 5(6)-carboxyfluorescein (5(6)-FAM). The sensor demonstrates reliable pH detection in biological tissues, showing potential for biomedical applications.

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    Area of Science:

    • Biomedical Engineering
    • Materials Science
    • Analytical Chemistry

    Background:

    • Accurate pH monitoring is crucial for biological systems.
    • Developing sensitive and biocompatible pH sensors remains a challenge.

    Purpose of the Study:

    • To develop and characterize a hydrogel optical fiber fluorescence pH sensor.
    • To evaluate its performance for pH detection in biological samples.

    Main Methods:

    • Fabrication of a hydrogel optical fiber using polyethylene glycol diacrylate (PEGDA) and 2-hydroxy-2-methylpropiophenone.
    • Doping the fiber core with the pH-sensitive dye 5(6)-carboxyfluorescein (5(6)-FAM).
    • Testing the sensor's response, linearity, repeatability, and reversibility across various pH ranges and in pork tissue.

    Main Results:

    • The sensor exhibited a good response in the pH range of 5.0-9.0.
    • A linear detection range of pH 6.0-8.0 was achieved with high linearity (R²=0.9904).
    • The sensor demonstrated good repeatability, reversibility, and a resolution of 0.07 pH units, with successful detection in pork tissues.

    Conclusions:

    • The hydrogel optical fiber pH sensor shows excellent performance characteristics.
    • Its biocompatibility and ion permeability make it suitable for biomedical applications.
    • This sensor offers a promising tool for in-situ pH monitoring in biological environments.