Related Experiment Video
Updated: Jul 9, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Digital holography without a dark room environment: extraction of interference fringes by using deep learning
Abstract:
When obtaining digital holograms, dark rooms are used to prevent the influence of natural light on the formation of holograms. Further, in recent years, researchers have actively studied machine learning techniques such as deep learning to resolve image-related problems. In this study, we obtained a pair of holograms influenced by natural light and holograms unaffected by natural light, and trained U-Net to perform image transformation to remove the effects of natural light from holograms. Thus, this study aimed to propose a method for eliminating the effects of natural light from holograms by using the U-Net we trained. To verify the effectiveness of the proposed method, we evaluated the image quality of the reconstructed image of holograms before and after image processing by U-Net. The results showed that the peak signal-to-noise ratio (PSNR) increased by 7.38 [dB] after processing by U-Net. Additionally, the structural similarity index (SSIM) increased by 0.0453 after processing by U-Net. This study confirmed that in digital holography, holograms can be acquired without the use of a dark room and that the method proposed in this study can eliminate the effects of natural light and produce high-quality reconstructed images.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy

