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Published on: January 18, 2014
Enhanced extracellular ammonium release in the plant endophyte Gluconacetobacter diazotrophicus through genome
Benjamin R Dietz1, Neil E Olszewski2, Brett M Barney1,3
1Department of Bioproducts and Biosystems Engineering, University of Minnesota , St. Paul, Minnesota, USA.
Importance:
Our results demonstrate increased extracellular ammonium release in the endophyte plant growth-promoting bacterium Gluconacetobacter diazotrophicus. Strains were constructed in a manner that leaves no antibiotic markers behind, such that these strains contain no transgenes. Levels of ammonium achieved by cultures of modified G. diazotrophicus strains reached concentrations of approximately 18 mM ammonium, while wild-type G. diazotrophicus remained much lower (below 50 µM). These findings demonstrate a strong potential for further improving the biofertilizer potential of this important microbe.
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