Quality Control of Mass-Encoded Nanodevices by Compartmented DNA Origami Frames for Precision Information Coding and...

Area of Science:

• Nanotechnology
• Biochemistry
• Information Security

Background:

• Encoded nanostructures offer multiplexed signaling for assays and security.
• Precise control over nanomaterial composition and structure for distinguishable signals is challenging.

Purpose of the Study:

• To propose a quality control methodology for constructing mass-encoded nanodevices (MNTs-TDOFs).
• To enable precise control over the arrangement and stoichiometry of mass nanotags (MNTs) within DNA origami frames (TDOFs).

Main Methods:

• Utilized mass spectrometry (MS) multiplexing capability and DNA origami spatial addressability.
• Engineered compartmented tetrahedral DNA origami frames (TDOFs) to precisely arrange four types of mass nanotags (MNTs).
• Developed a quality control methodology for constructing MNTs-TDOFs with customized MS patterns.

Main Results:

• Achieved fine regulation of MNT arrangement and stoichiometry, generating characteristic MS patterns.
• Demonstrated programmability of MNTs and orthogonality of compartments for static tagging and dynamic nanoprobes.
• Established a high-capacity coding system for secure information encryption and decryption with structure control at the single TDOF level.
• Mapped logic circuits and cell surface signaling events (c-Met recognition and dimerization) using MS interrogation.

Conclusions:

• The developed MNTs-TDOFs provide a robust platform for multiplexed assays and secure information encryption.
• The methodology ensures reproducible and distinguishable signals, overcoming previous limitations in nanomaterial coding.
• The nanodevices show potential for advanced applications in sensing, logic operations, and biological signaling analysis.