Related Experiment Video
Updated: Jul 29, 2026
00:07
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.3K
Comprehensive Long-Read Sequencing Analysis Discloses the Transcriptome Features of Papillary Thyroid Microcarcinoma
Yanqiang Wang1, Binbin Zou1, Yanyan Zhang2
1Key Laboratory of Cellular Physiology of the Ministry of Education (Shanxi Medical University), Translational Medicine Research Center, Department of Pathology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
The Journal of Clinical Endocrinology and Metabolism
|December 1, 2023
Summary
Researchers characterized the transcriptome of papillary thyroid microcarcinoma (PTMC), identifying novel isoforms linked to lymph node metastasis. This discovery offers insights into PTMC aggressiveness and potential prognostic markers.
Area of Science:
- Molecular Biology
- Genomics
- Oncology
Background:
- Papillary thyroid microcarcinoma (PTMC) is the most prevalent thyroid cancer subtype.
- Lymph node metastasis in PTMC correlates with poorer patient prognosis.
Purpose of the Study:
- To comprehensively map the PTMC transcriptome.
- To identify specific transcripts associated with lateral neck lymph node metastasis (pN1b subtype).
Main Methods:
- Full-length transcriptome sequencing was performed on 64 PTMC samples.
- Bioinformatic analyses were used for transcript characterization, annotation, and functional prediction (HMMER, DeepLoc, DeepTMHMM).
- Differential gene expression analysis identified candidate transcripts linked to metastasis.
Main Results:
- Alternative splicing, particularly exon skipping (27.82%), was prevalent.
- A significant proportion of transcripts (42.56%) were novel isoforms.
- Novel isoforms of FRMD3, NOD1, and SHROOM4 were significantly associated with the pN1b subtype.
Conclusions:
- The study presents a global view of the PTMC transcriptome.
- Novel transcript isoforms associated with PTMC aggressiveness and metastasis were identified.
