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Long Non-coding RNA SNHG1 Suppresses the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Binding

Kaihua Pan1, Yuanyuan Lu1, Daning Cao1

  • 1Department of Orthopaedics, The First Hospital of Changsha, No. 311, Yingpan Road, Kaifu District, Changsha, 410005, Hunan, People's Republic of China.

Biochemical Genetics
|December 1, 2023
PubMed

Insights

This study reveals that SNHG1 suppresses bone formation in osteoporosis by activating pyroptosis via HMGB1 interaction. Targeting SNHG1 may offer a novel therapeutic strategy for osteoporosis treatment.

Area of Science:

  • Biomedical Research
  • Molecular Biology
  • Cell Biology

Background:

  • Osteoporosis (OP) poses a significant health burden on the elderly, with current treatments having limited long-term efficacy.
  • Identifying novel therapeutic targets for OP is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the role of SNHG1 in osteogenic differentiation and its potential as a therapeutic target for osteoporosis.
  • To elucidate the molecular mechanisms underlying SNHG1's function in bone metabolism.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blotting to measure gene and protein expression.
  • Enzyme-linked immunosorbent assay (ELISA) for cytokine detection.
  • RNA pull-down assay to confirm molecular interactions.
  • Alizarin red staining to assess osteogenic differentiation.

Main Results:

  • SNHG1 and HMGB1 were upregulated in osteoporosis patients' serum and inhibited osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
  • SNHG1 promotes HMGB1 expression, activates pyroptosis, and suppresses osteogenic differentiation.
  • Silencing HMGB1 or inhibiting caspase-1 partially reversed SNHG1's inhibitory effects on osteogenesis.

Conclusions:

  • SNHG1 suppresses osteogenic differentiation by activating pyroptosis through interaction with and promotion of HMGB1 expression.
  • SNHG1 represents a potential therapeutic target for osteoporosis.
  • This study reveals SNHG1's novel role in regulating osteogenic differentiation via pyroptosis.
Keywords:
HMGB1Osteogenic differentiationOsteoporosisPyroptosisSNHG1

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