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A New Affinity-Based Probe to Profile MMP Active Forms.

Carole Malgorn1, François Becher2, Pierrick Bruyat1

  • 1Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SIMoS, Gif-sur-Yvette, France.

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Summary

New affinity-based probes (AfBPs) enable detection of active matrix metalloproteinases (MMPs) in complex biological samples without photoactivation. This method allows for sensitive identification of MMPs in various systems, including in vivo.

Keywords:
Affinity-based probesMass spectrometryMatrix metalloproteinasesStreptavidin-based enrichment

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Area of Science:

  • Biochemistry
  • Proteomics
  • Chemical Biology

Background:

  • Matrix metalloproteinases (MMPs) are crucial enzymes involved in numerous physiological and pathological processes.
  • Accurate detection of active MMPs in complex biological environments remains a challenge.
  • Existing methods often require external triggers like photoactivation, limiting their applicability.

Purpose of the Study:

  • To develop a novel affinity-based probe (AfBP) for labeling and identifying active matrix metalloproteinases (MMPs).
  • To establish a photoactivation-free method for MMP detection in complex proteomes.
  • To demonstrate the utility of the developed AfBP in diverse biological systems, including in vivo.

Main Methods:

  • Development of a new generation of affinity-based probes (AfBPs).
  • Proximity-driven, photoactivation-free reaction between AfBP and active MMPs.
  • Streptavidin-based enrichment of biotin-tagged MMPs.
  • On-beads tryptic digestion followed by mass spectrometry analysis for MMP signature peptide identification.

Main Results:

  • Successful labeling and identification of active MMPs in complex proteomes.
  • Demonstration of a proximity-driven reaction that does not require external triggers.
  • Effective enrichment and mass spectrometry-based identification of MMPs.
  • Applicability of the method to detect multiple MMPs across various biological systems.

Conclusions:

  • The developed affinity-based probes offer a sensitive and versatile tool for detecting active MMPs.
  • This photoactivation-free approach simplifies MMP detection and broadens its application scope.
  • The method is suitable for analyzing MMP activity in complex biological matrices and in vivo conditions.