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Related Experiment Video

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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
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Genetically Engineered Protein-Based Bioadhesives with Programmable Material Properties.

Juya Jeon1, Kok Zhi Lee1, Xiaolu Zhang1

  • 1Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, One Brookings Drive, Saint Louis, Missouri 63130, United States.

ACS Applied Materials & Interfaces
|December 1, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed tunable silk-amyloid-mussel foot protein (SAM) hydrogels for surgical repair. Modifying protein sequences controlled hydrogel properties, enhancing strength and underwater adhesion for advanced bioadhesives.

Keywords:
amyloid beta-peptidesbioadhesivemussel foot proteinprogrammable material propertiesprotein materialssynthetic biologyunderwater adhesive

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Area of Science:

  • Biomaterials Science
  • Protein Engineering
  • Tissue Engineering

Background:

  • Silk-amyloid-mussel foot protein (SAM) hydrogels offer a unique combination of tunable properties, biocompatibility, and strong underwater adhesion.
  • Understanding the relationship between protein sequence and hydrogel characteristics is crucial for designing effective surgical bioadhesives.

Purpose of the Study:

  • To fabricate and characterize SAM hydrogels with varying silk-amyloid repeat lengths and mussel foot protein (Mfp) lengths.
  • To elucidate the sequence-structure-property relationships governing SAM hydrogel performance.

Main Methods:

  • Fabrication of SAM hydrogels using fusion proteins with controlled silk-amyloid repeats and Mfp lengths.
  • Characterization of hydrogel structure (e.g., β-sheet content) and mechanical properties (e.g., cohesive strength, toughness, ultimate strength, ultimate strain).
  • Assessment of underwater adhesion to biological surfaces (porcine skin).

Main Results:

  • Increasing silk-amyloid repeats significantly enhanced β-sheet content, leading to improved cohesive strength and toughness.
  • Extending Mfp length beyond a certain point decreased β-sheet content but enhanced surface adhesion.
  • A specific variant (16xKLV-2Mfp) demonstrated high ultimate strength (3.0 MPa), strain (664%), and underwater adhesion (416 kPa).

Conclusions:

  • The study establishes clear sequence-structure-property relationships for SAM hydrogels.
  • These findings provide a foundation for designing customized protein-based adhesives for surgical applications.
  • Optimized SAM hydrogels show promise as advanced bioadhesives for tissue repair.