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Related Experiment Video

Updated: Jul 9, 2025

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

PIM kinases regulate early human Th17 cell differentiation.

Tanja Buchacher¹, Ankitha Shetty², Saara A Koskela³

¹Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland; InFLAMES Research Flagship Center, University of Turku, 20520 Turku, Finland.

|December 1, 2023

Summary

PIM kinases inhibit human T helper 17 (Th17) cell differentiation. PIM deficiency promotes Th17 gene expression, suggesting a role for PIMs in autoimmune diseases.

Keywords:

CP: Immunology PIM kinases T helper cell differentiation Th1 cells Th17 cells bulk RNA sequencing single-cell RNA sequencing transcriptomics

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Last Updated: Jul 9, 2025

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Published on: October 25, 2024

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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

PIM kinases (PIM1, PIM2, PIM3) are implicated in tumorigenesis and cytokine signaling in immune-mediated diseases.
Uncontrolled T helper 17 (Th17) cell activation is linked to autoimmune pathogenesis.
The precise role of PIM kinases in human Th17 cell regulation remains largely uncharacterized.

Purpose of the Study:

To investigate the molecular function of PIM kinases in regulating early human Th17 cell differentiation.
To elucidate how PIM kinases influence transcriptional gene regulation during Th17 cell development.

Main Methods:

Combined PIM triple knockdown with bulk and single-cell RNA sequencing (scRNA-seq).
Analyzed transcriptional changes in gene expression during early human Th17 cell differentiation.

Main Results:

PIM deficiency enhanced the early expression of key Th17-related genes.
PIM deficiency suppressed the expression of Th1-lineage genes.
PIM kinases modulate T helper cell signaling, potentially through STAT1 and STAT3 pathways.

Conclusions:

PIM kinases play an inhibitory role in human Th17 cell differentiation.
The findings suggest a potential association between PIM kinase activity and autoimmune phenotypes.