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Multifunctional chitosan-based gel sponge with efficient antibacterial, hemostasis and strong adhesion
Lanfang Sun1, Junyi Zhou1, Jieying Lai1
1DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
International Journal of Biological Macromolecules
|December 1, 2023
Summary
A novel chitosan-based gel sponge (CTMG) demonstrates strong adhesion and effective hemostasis for wound dressings. This material shows promising antibacterial and biocompatible properties for clinical applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Developing advanced wound dressings with effective hemostatic and antibacterial properties is crucial for clinical practice.
- Existing hemostatic agents often face challenges related to adhesion, biocompatibility, and infection prevention.
- Chitosan-based materials offer potential due to their inherent properties, but require modification for enhanced performance.
Purpose of the Study:
- To develop and characterize a novel chitosan-based gel sponge with enhanced adhesive and hemostatic capabilities.
- To evaluate the antibacterial activity and biocompatibility of the developed material.
- To assess the efficacy of the chitosan-based gel sponge in controlling bleeding in vivo and in vitro.
Main Methods:
- Preparation of tris(hydroxymethyl) methyl glycine-modified chitosan derivative (CTMG) and subsequent freeze-drying to form a multi-hollow gel sponge.
- Assessment of adhesion strength on porcine skin and mechanical properties.
- Evaluation of bacteriostatic activity through assays, and biocompatibility via cytotoxicity and hemolytic assays.
- In vitro and in vivo hemostasis experiments, including liver hemorrhage model in rats.
Main Results:
- The CTMG gel sponge exhibited significant adhesion strength (113 KPa) on porcine skin, surpassing fibrin glue.
- Demonstrated effective bacteriostatic properties and excellent biocompatibility.
- Showcased superior hemostatic efficacy in both in vitro and in vivo models compared to existing hemostatic sponges.
- The material proved effective in controlling liver hemorrhage in a rat model.
Conclusions:
- The developed CTMG gel sponge possesses robust adhesive, hemostatic, antibacterial, and biocompatible properties.
- This novel chitosan-based material shows significant potential as an advanced wound dressing and hemostatic agent.
- CTMG gel sponge is a promising candidate for diverse clinical applications in wound management and hemorrhage control.
