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Updated: Jul 9, 2025
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Oxidized high-density lipoprotein associates with atrial fibrillation
Nikolaos Pagonas1, Rhea Mueller2, Linda Weiland3
1Department of Cardiology, University Hospital Ruppin-Brandenburg, Medical School Theodor Fontane, Neuruppin, Germany; Faculty of Health Sciences, Joint Faculty of the Brandenburg University of Technology Cottbus-Senftenberg, The (MHB) Theodor Fontane and the University of Potsdam, Potsdam, Germany.
Insights
Reduced antioxidant function in high-density lipoprotein (HDL) is linked to atrial fibrillation (AF). This finding suggests impaired HDL function may contribute to the development of this common heart arrhythmia.
Area of Science:
- Cardiology
- Biochemistry
- Molecular Medicine
Background:
- Atrial fibrillation (AF) is a prevalent chronic arrhythmia linked to increased mortality.
- Emerging evidence suggests a connection between inflammation, oxidative stress, and AF.
- Dysfunctional high-density lipoprotein (HDL) may play a role in AF development due to its impact on cardiomyocytes.
Purpose of the Study:
- To investigate the association between reduced HDL antioxidant function and the presence of AF.
Main Methods:
- A multicenter cross-sectional study involving 1206 participants.
- Participants were categorized into AF (n=233) and no AF (n=973) groups.
- HDL antioxidant function was assessed using a cell-free assay measuring normalized HDL lipid peroxide content (nHDLox).
Main Results:
- Individuals with AF exhibited a 9% higher mean relative nHDLox compared to those without AF (P=.025).
- nHDLox showed a significant association with AF across logistic regression models, even after adjusting for key risk factors (all P≤.01).
Conclusions:
- Impaired HDL antioxidant function is associated with the presence of AF.
- This supports the hypothesis that lipoprotein dysfunction contributes to the electrophysiological changes in cardiomyocytes seen in AF.
- Elevated nHDLox is identified as a contributing factor to AF initiation and progression.
Background:
Atrial fibrillation (AF) is the most common heart arrhythmia and considered to be a progressive chronic disease associated with increased morbidity and mortality. Recent data suggest a link between inflammation, oxidative stress, and AF, although the underlying mechanisms are not fully understood. Because oxidized lipoproteins cause structural damage and electrophysiologic changes in cardiomyocytes, it is feasible that the transformation of atheroprotective high-density lipoprotein (HDL) into dysfunctional HDL contributes to the development of AF.
Objective:
The purpose of this study was to determine whether a reduced antioxidant function of HDL is associated with the presence of AF.
Methods:
In this multicenter cross-sectional cohort study, we assessed HDL function in sera of 1206 participants. Patients were divided into groups according to the presence of AF (n = 233) or no AF (n = 973). A validated cell-free biochemical assay was used to determine reduced HDL antioxidant function as assessed by increased normalized HDL lipid peroxide content (nHDLox).
Results:
Participants with AF had a 9% higher mean relative nHDLox compared to persons without AF (P = .025). nHDLox was strongly associated with AF in all models of logistic regression, including the analysis adjusted for age, sex, and risk factors for AF (all P ≤.01).
Conclusion:
Reduced antioxidant HDL function is associated with the presence of AF, which supports growing evidence that impaired lipoprotein function is linked to electrophysiological changes in cardiomyocytes. nHDLox is one of several contributors to the initiation and perpetuation of AF.
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