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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism

Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger

    Optics Express
    |December 2, 2023
    PubMed
    Summary

    A novel hexagonal prism telescope allows seamless adjustment of laser spot separation in super-resolution microscopy. This innovation enhances spatial resolution and enables transitions to total internal reflection fluorescence imaging.

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