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Updated: Jul 9, 2025
Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord
Published on: February 26, 2019
Characterization of synaptic structural plasticity in mouse spinal dorsal horn neurons
Qin Ru1, Jennifer Magnusson2, Lingyong Li3
1Department of Health and Kinesiology, School of Physical Education, Jianghan University, Wuhan 430056, China; Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
Abstract:
Here, we present a pipeline for the characterization of synaptic structural plasticity in mouse spinal dorsal horn (SDH) neurons. We describe steps for the intra-SDH microinjection of the EGFP virus to sparsely label L4 SDH neurons without laminectomy, wide dynamic range neuron imaging, dendritic spine morphometric analysis, and F-actin to G-actin ratio measurement. This protocol can be applied to investigate the synaptic structural plasticity mechanisms in the SDH as well as in the brain. For complete details on the use and execution of this protocol, please refer to Li et al. (2023).1.
