Related Experiment Videos
Protocol to image and analyze hippocampal network dynamics in non-anesthetized mouse pups
Mirindra R Ratsifandrihamanana1, Robin F Dard1, Julien Denis1
1Turing Centre for Living Systems, Aix-Marseille University, INSERM, INMED U1249, France.
STAR Protocols
|December 2, 2023
Summary
This study presents a protocol for two-photon calcium imaging in developing mouse pups, enabling the analysis of neural circuit dynamics during early life. The methods cover surgery, imaging, and data analysis for developmental neuroscience research.
Area of Science:
- Neuroscience
- Developmental Biology
- Imaging Techniques
Background:
- Two-photon calcium imaging advances understanding of neural circuits.
- Imaging neural activity during development is technically challenging.
Purpose of the Study:
- Provide a protocol for imaging CA1 neurons in mouse pups.
- Develop a data analysis pipeline for developmental calcium imaging data.
Main Methods:
- Intracerebroventricular injection in mouse pups.
- Cranial window surgery for brain access.
- Two-photon calcium imaging acquisition.
- Data analysis pipeline for imaging data.
Main Results:
- Successful imaging of CA1 neurons in developing mice.
- Established a comprehensive analysis pipeline for developmental imaging data.
- Facilitated data sharing for developmental neuroscience studies.
Conclusions:
- The protocol enables advanced neural circuit analysis during early development.
- The analysis pipeline supports robust interpretation of developmental imaging data.
- This work provides a foundation for future developmental neuroscience research using calcium imaging.