A sensing platform for ascorbic acid based on the spatial confinement of covalent organic frameworks
Jierui Yang1, Huiting Qiu1, Shuang Meng1
1College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|December 2, 2023
Summary
A novel covalent organic framework, TpNda-COF, mimics enzymes to detect ascorbic acid (AA). This light-activated system offers a sensitive and stable method for quantifying AA, crucial for human health.
Area of Science:
- Materials Science
- Biomimetic Chemistry
- Analytical Chemistry
Background:
- Ascorbic acid (AA) is vital for human health and physiological processes.
- Enzyme mimics offer potential for sensitive and stable analytical platforms.
- Covalent organic frameworks (COFs) provide unique structural properties for catalysis.
Purpose of the Study:
- To synthesize and characterize a TpNda-COF material.
- To develop a light-activated, enzyme-mimicking system for ascorbic acid detection.
- To evaluate the selectivity, sensitivity, and detection limits of the proposed sensing platform.
Main Methods:
- Synthesis of TpNda-COF from Tp and Nda.
- Utilizing TpNda-COF as a mimic oxidase under purple light (405 nm).
- Quantifying ascorbic acid via UV-Vis spectrophotometry by monitoring TMB oxidation and reduction.
Main Results:
- TpNda-COF exhibited excellent oxidase-like activity, catalyzing TMB oxidation.
- The system demonstrated a significant color change upon ascorbic acid introduction, enabling detection.
- Achieved a wide linear detection range (5–50 μM) with a low detection limit (1.44 μM).
Conclusions:
- The TpNda-COF mimic oxidase provides a stable, efficient, and light-controlled platform for ascorbic acid detection.
- This method is cost-effective and shows high selectivity and sensitivity.
- The developed sensing strategy holds promise for practical applications in ascorbic acid analysis.
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