Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interlaboratory comparison with a reference measurement procedure (RMP) for determining 24,25-dihydroxyvitamin D<sub>3</sub> in human serum using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Analytical and bioanalytical chemistry·2026
Same author

Transferability and Between Laboratory Imprecision of an LC-MS/MS-Based Reference Measurement Procedure for Apolipoproteins.

Clinical chemistry·2026
Same author

Current Challenges and Needs of Clinical Laboratories in the Asia-Pacific Region: A Cross-Country Assessment.

The journal of applied laboratory medicine·2026
Same author

Using Aggregated Proficiency Testing Results to Identify Systematic Error.

The journal of applied laboratory medicine·2025
Same author

Demonstrating commutability of an existing certified reference material for use with an end-user measurement procedure that was not included in the original commutability assessment.

Clinica chimica acta; international journal of clinical chemistry·2025
Same author

Challenges in developing accurate assays for the measurement of estradiol and testosterone in postmenopausal women.

Menopause (New York, N.Y.)·2025
Same journal

Evaluation of hemoglobin interference thresholds for chemical urinalysis panels.

Clinical biochemistry·2026
Same journal

Integrated genomic and biochemical diagnosis of a novel homozygous start-loss variant in AKR1D1 associated with neonatal cholestasis.

Clinical biochemistry·2026
Same journal

Agreement between POC glucose meters and blood gas analyzers varies across ICU patient populations.

Clinical biochemistry·2026
Same journal

From variability to value: Advancing vancomycin therapeutic drug monitoring toward decision-grade practice through commutability-informed external quality assessment and uncertainty-aware reporting.

Clinical biochemistry·2026
Same journal

Response to the letter to the editor: From variability to value: Advancing vancomycin therapeutic drug monitoring toward decision-grade practice through commutability-informed external quality assessment and uncertainty-aware reporting.

Clinical biochemistry·2026
Same journal

What are the correlates of laboratory productivity in clinical laboratories in the Asia Pacific region?

Clinical biochemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 9, 2025

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

13.2K

Accuracy-based proficiency testing for estradiol measurements.

Zhimin Tim Cao1, Robert Rej2, Hubert Vesper3

  • 1Wadsworth Center, New York State Department of Health, Albany, NY, United States; Department of Pathology, SUNY Upstate Medical University, Syracuse, NY, United States.

Clinical Biochemistry
|December 3, 2023
PubMed
Summary
This summary is machine-generated.

Accuracy-based proficiency testing (PT) is crucial for reliable estradiol measurements, especially at low concentrations. Conventional PT methods may not accurately assess accuracy with non-commutable samples, highlighting the need for improved assessment strategies.

Keywords:
AccuracyEstradiolProficiency testingStandardization

More Related Videos

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency
09:49

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency

Published on: September 11, 2016

14.1K
Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
08:56

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

Published on: April 7, 2023

908

Related Experiment Videos

Last Updated: Jul 9, 2025

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

13.2K
Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency
09:49

Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency

Published on: September 11, 2016

14.1K
Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
08:56

Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

Published on: April 7, 2023

908

Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Endocrinology

Background:

  • Accurate estradiol measurements are vital for clinical diagnostics and research.
  • Conventional proficiency testing (PT) may not adequately assess accuracy, particularly with non-commutable samples.
  • Method peer-group means, often used as targets in PT, can obscure true method accuracy.

Purpose of the Study:

  • To evaluate the accuracy of estradiol measurements using an accuracy-based proficiency testing approach.
  • To assess the performance of various analytical systems and laboratories in measuring estradiol.
  • To highlight the limitations of conventional PT and advocate for accuracy-based assessment.

Main Methods:

  • Five serum samples from single donors were distributed to 76 certified laboratories.
  • Laboratories analyzed samples for estradiol, with biases assessed against CDC-defined targets.
  • Performance was evaluated using criteria from NYSDOH, CLIA, and biological variation (BV).

Main Results:

  • Significant positive biases were observed for common analytical systems at low estradiol concentrations.
  • Only a fraction of analytical systems met the Hormone Standardization Program (HoSt) criterion at low concentrations.
  • While most laboratories would pass conventional PT, accuracy-based assessment revealed substantial measurement variability.

Conclusions:

  • There is a clear need to improve the accuracy of estradiol measurements, particularly at low concentrations.
  • Accuracy-based PT provides a more definitive evaluation of method and analytical system performance.
  • Implementing accuracy-based PT is essential for ensuring reliable estradiol testing.