Related Experiment Video
Updated: Jul 9, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
Asiaticoside Down-Regulates HIF-1α to Inhibit Proliferation, Migration, and Angiogenesis in Thyroid Cancer Cells
Yi Zhang1, Yifan Han1, Jianda Dong1
1Department of Neck Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
Background:
Thyroid cancer (TC), the most prevalent endocrine malignancy, has been subjected to various treatment methods. However, the efficacy of asiaticoside (AC) for treating TC remains uncertain.
Aims:
To explore the impact of AC on TC and determine its potential mechanisms of action.
Study Design:
In vitro and in vivo cell line study.
Methods:
We evaluated the effects of AC on human TC cell lines, namely TPC-1 and FTC-133. Both in vitro and in vivo experimental validations were conducted.
Results:
AC significantly diminished the viability and proliferation of TC cells based on the CCK-8 assay and Edu staining findings. Migration and invasion assays revealed that AC effectively curtailed the migration and invasiveness of TC cells. The tube formation assay demonstrated that AC substantially impeded TC cell-induced angiogenesis. Western blot assay revealed that AC significantly reduced the expression levels of TRAF6, HIF-1α, and VEGFA, indicating that AC could potentially exert its anticancer effect by inhibiting the TRAF6/HIF1α pathway. Our in vivo experiments, which involved administering AC to BALB/c nude mice injected with TPC-1 cells, demonstrated significant inhibition of tumor growth and reduction in the expression of Ki-67, TRAF6, HIF-1α, and VEGFA.
Conclusion:
Our study highlights the significant inhibitory effect of AC on TC, offering fresh insights and potential drug candidates for TC treatment.
Insights
Asiaticoside (AC) significantly inhibits thyroid cancer (TC) cell viability, proliferation, migration, and angiogenesis. This study reveals AC
Area of Science:
- Oncology
- Endocrinology
- Pharmacology
Background:
- Thyroid cancer (TC) is the most common endocrine malignancy.
- Current TC treatments have limitations, and the efficacy of asiaticoside (AC) is under investigation.
Purpose of the Study:
- To investigate the anti-cancer effects of asiaticoside (AC) on thyroid cancer (TC).
- To elucidate the underlying molecular mechanisms of AC action in TC.
Main Methods:
- In vitro studies using human TC cell lines (TPC-1, FTC-133).
- In vivo experiments in BALB/c nude mice xenograft models.
- Assays included CCK-8, Edu staining, migration, invasion, tube formation, and Western blot.
Main Results:
- AC reduced TC cell viability, proliferation, migration, and angiogenesis.
- AC inhibited tumor growth in vivo.
- AC downregulated TRAF6, HIF-1α, and VEGFA expression, suggesting inhibition of the TRAF6/HIF1α pathway.
Conclusions:
- Asiaticoside (AC) demonstrates significant anti-thyroid cancer properties.
- AC shows potential as a therapeutic agent for thyroid cancer treatment.
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
01:20Synthesis and Regulation of Thyroid Hormones
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
02:34Inhibition of Cdk Activity
02:38Mitogens and the Cell Cycle
01:15Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
01:18Functions of Thyroid Hormones
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...