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Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Related Experiment Video

Updated: Jun 15, 2026

Fabrication of White Light-emitting Electrochemical Cells with Stable Emission from Exciplexes

Fabrication of White Light-emitting Electrochemical Cells with Stable Emission from Exciplexes

Published on: November 15, 2016

Engineering luminopsins with improved coupling efficiencies.

Ashley Slaviero^1,2, Nipun Gorantla¹, Jacob Simkins¹

¹Central Michigan University, College of Medicine, Mount Pleasant, Michigan, United States.

Biorxiv : the Preprint Server for Biology

|December 4, 2023

Summary

This summary is machine-generated.

Brighter luciferases and more sensitive opsins enhance luminopsin (LMO) efficacy for neuronal control. N-terminal fusions are superior, guiding future LMO development for optogenetics and chemogenetics.

Keywords:

Förster resonance energy transfer bioluminescence luciferase opsin optogenetics whole cell patch clamp recording

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Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

Area of Science:

Neuroscience
Molecular Biology
Biotechnology

Background:

Luminopsins (LMOs) integrate bioluminescence and optogenetics for dual neuronal control.
Understanding LMO design principles is crucial for optimizing these tools.

Approach:

Evaluated impact of luciferase brightness, opsin sensitivity, spectral pairing, and fusion arrangement on LMO function.
Quantified LMO efficacy using whole-cell patch clamp in HEK293 cells and MEA recordings in primary neurons.

Key Points:

Luciferase brightness and opsin sensitivity significantly influence LMO performance.
N-terminal luciferase-opsin fusions demonstrate superior efficacy over C-terminal or multi-terminal designs.
Precise spectral overlap between luciferase emission and opsin absorption is less critical than previously thought.

Conclusions:

Optimizing LMOs involves combining brighter bioluminescent components with more photosensitive opsins.
Future advancements may leverage molecular evolution of fusion proteins, potentially utilizing FRET mechanisms for enhanced bioluminescent optogenetic tools.