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Determination of 25-hydroxyvitamin D3 in sheep tissues by high-performance liquid chromatography
Abstract:
A high-performance liquid chromatographic (HPLC) method is described for the determination of 25-hydroxyvitamin D3 (25-OH-D3) in sheep tissue. The procedure involved saponification followed by chromatography on Sephadex LH-20 in order to remove extraneous material. Quantitation of 25-OH-D3 in tissue samples containing as little as 2 ng/g, was achieved by HPLC and ultraviolet detection at 254 nm in HPLC system.
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