Pseudomonas aestuarii sp. nov., isolated from tidal flat sediment

Two novel Pseudomonas strains, SA3-5^T and SA3-6, were isolated from a tidal flat (getbol) in the Republic of Korea. Strains SA3-5^T and SA3-6 were subjected to polyphasic characterization to determine their taxonomic affiliations. Cells were Gram-stain-negative, aerobic, rod-shaped and motile by using peritrichous flagella. Based on their 16S rRNA gene sequences, strains SA3-5^T and SA3-6 exhibited a high degree of similarity (100 %) and were classified within the genus Pseudomonas. Furthermore, the closest related species to SA3-5^T and SA3-6 were Pseudomonas taeanensis MS-3^T (98.3 %). The ranges of average nucleotide identity and digital DNA-DNA hybridization values between SA3-5^T and closely related species were 75.9-89.1% and 21.3-38.7%, respectively, both of which being below the thresholds for delineating novel strains. Strain SA3-5^T and SA3-6 contained C_16 : 1 ω6с and/or C_16 : 1 ω7с (summed feature 3), C_16 : 0 and C_18 : 1 ω6с and/or C_18 : 1 ω7с (summed feature 8) as the major fatty acids. The predominant respiratory quinone was Q-9. The DNA G+C content of strain SA3-5^T was 62.5 mol%. Based on their combined phenotypic, chemotaxonomic and phylogenetic characterisitics, strains SA3-5^T and SA3-6 represent a novel species of the genus Pseudomonas for which the name Pseudomonas aestuarii sp. nov. is proposed. The type strain is SA3-5^T (=KCTC 92395^T=JCM 35697^T).