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CsPM5.2, a phosphate transporter protein-like gene, promotes powdery mildew resistance in cucumber
Jingxian Sun1,2, Jingtao Nie1,3, Tingting Xiao1
1School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.
Abstract:
Powdery mildew (PM) is one of the most serious fungal diseases affecting cucumbers (Cucumis sativus L.). The mechanism of PM resistance in cucumber is intricate and remains fragmentary as it is controlled by several genes. In this study, we detected the major-effect Quantitative Trait Locus (QTL), PM5.2, involved in PM resistance by QTL mapping. Through fine mapping, the dominant PM resistance gene, CsPM5.2, was cloned and its function was confirmed by transgenic complementation and natural variation identification. In cultivar 9930, a dysfunctional CsPM5.2 mutant resulted from a single nucleotide polymorphism in the coding region and endowed susceptibility to PM. CsPM5.2 encodes a phosphate transporter-like protein PHO1; H3. The expression of CsPM5.2 is ubiquitous and induced by the PM pathogen. In cucumber, both CsPM5.2 and Cspm5.1 (Csmlo1) are required for PM resistance. Transcriptome analysis suggested that the salicylic acid (SA) pathway may play an important role in CsPM5.2-mediated PM resistance. Our findings help parse the mechanisms of PM resistance and provide strategies for breeding PM-resistant cucumber cultivars.
Insights
Researchers identified the CsPM5.2 gene, crucial for powdery mildew (PM) resistance in cucumbers. This gene, encoding a phosphate transporter, is essential for plant defense and offers strategies for developing disease-resistant cucumber varieties.
Area of Science:
- Plant Pathology
- Molecular Genetics
- Crop Science
Background:
- Powdery mildew (PM) is a major fungal disease impacting cucumber production.
- Understanding the genetic basis of PM resistance is vital for crop improvement.
- Existing knowledge on cucumber PM resistance mechanisms is incomplete.
Purpose of the Study:
- To identify and clone major genes conferring resistance to powdery mildew in cucumber.
- To elucidate the function and mechanism of the identified resistance gene.
- To provide insights for breeding PM-resistant cucumber cultivars.
Main Methods:
- Quantitative Trait Locus (QTL) mapping to detect PM resistance loci.
- Fine mapping and gene cloning to isolate the resistance gene CsPM5.2.
- Transgenic complementation and natural variation analysis to confirm gene function.
Main Results:
- A major-effect QTL, PM5.2, was identified, and the dominant resistance gene CsPM5.2 was cloned.
- A single nucleotide polymorphism in dysfunctional CsPM5.2 caused PM susceptibility in cultivar 9930.
- CsPM5.2 encodes a phosphate transporter-like protein (PHO1;H3) and its expression is induced by the pathogen.
- Both CsPM5.2 and Cspm5.1 are necessary for PM resistance, with the salicylic acid pathway implicated.
Conclusions:
- The cloned gene CsPM5.2 is a key determinant of powdery mildew resistance in cucumber.
- Dysfunctional CsPM5.2 leads to susceptibility, highlighting its critical role in defense.
- Findings provide a foundation for genetic strategies to enhance cucumber resistance to powdery mildew.
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