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Updated: Jul 9, 2025

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Glycerol as substrate and NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase enable higher production of
Raquel Salgado Batista1, Gabriel Luz Chaves1, Davi Benedito Oliveira1
1Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís, km 235, São Carlos, São Paulo 13565-905, Brazil.
Abstract:
Microbial engineering is a promising way to produce3-HP using biorenewable substrates such as glycerol. However, theglycerol pathway to obtain 3-HPrequires vitamin B-12, which hinders its economic viability. The present work showed that 3-HP can be efficiently produced from glycerol through the β-alanine pathway. To develop a cell factory for this purpose, glycerol was evaluated as a substrate and showed more than two-fold improved 3-HP production compared to glucose. Next, the reducing power was modulated by overexpression of an NADP+ -dependent glyceraldehyde-3-phosphate dehydrogenase coupled with CRISPR-based repression of the endogenous gapA gene, resulting in a 91 % increase in 3-HP titer. Finally, the toxicity of 3-HP accumulation was addressed by overexpressing a putative exporter (YohJK). Fed-batch cultivation of the final strain yielded 72.2 g/L of 3-HP and a productivity of 1.64 g/L/h, which are the best results for the β-alanine pathway and are similar to those found for other pathways.
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