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Manganese oxidation by Leptothrix discophora.

F C Boogerd, J P de Vrind

    Journal of Bacteriology
    |February 1, 1987
    PubMed
    Summary

    Leptothrix discophora SS1 bacteria release manganese-oxidizing factors that facilitate manganese oxidation. These factors, primarily a 110,000-Mr protein, are sensitive to various inhibitors and associated with MnO2 aggregates.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Environmental Science

    Background:

    • Leptothrix discophora SS1 is known to precipitate manganese oxides.
    • Understanding the mechanisms of microbial manganese oxidation is crucial for biogeochemical cycling.

    Purpose of the Study:

    • To identify and characterize the Mn2+-oxidizing factors released by Leptothrix discophora SS1.
    • To investigate the optimal conditions for manganese oxidation by these factors.

    Main Methods:

    • Batch culture growth of L. discophora SS1.
    • Manganese oxidation assays at varying pH.
    • Sensitivity testing of activity against heat, chemicals, and enzymes.
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein analysis.

    Main Results:

    • Optimal Mn2+ oxidation occurred at pH 7.5, buffered with HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid).
    • The Mn2+-oxidizing activity was inhibited by heat, phosphate, Tris, sodium azide (NaN3), mercuric chloride (HgCl2), sodium chloride (NaCl), sodium dodecyl sulfate, and pronase.
    • SDS-PAGE revealed a predominant 110,000-Mr protein band associated with Mn2+-oxidizing activity.
    • Manganese oxidation consumed O2 and released protons, with factors associated with MnO2 aggregates.

    Conclusions:

    • Leptothrix discophora SS1 releases soluble Mn2+-oxidizing factors, primarily a 110,000-Mr protein.
    • These factors are enzymes sensitive to various inhibitors, indicating a proteinaceous nature.
    • The findings contribute to understanding microbial mediation of manganese biogeochemistry.

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