Related Experiment Video
Updated: Jul 9, 2025
10:38
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
14.0K
A tuning fork-shaped bisbenzothiazole derivative as a pH-responsive digital fluorescent probe and its ex vivo
Priya Ranjan Sahoo1, Nikhil Kumar2, Keloth Sairam1
1Department of Chemistry, St. Stephen's College, University Enclave, Delhi, 110007, India. satish@ststephens.edu.
Analytical Methods : Advancing Methods and Applications
|December 5, 2023
Abstract:
A new highly emissive pH-responsive near-IR active digital probe was designed and synthesized. The probe is based on a bisbenzothiazole motif with a highly vulnerable hydrogen unit attached in an intramolecular fashion. The probe produced a large Stokes shift which was observed to be highly pH dependent. The optical pH dependence can be used for sensing pH over a wide range.
Related Concept Videos
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
01:14Photoluminescence: Applications
405
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
405