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Updated: Jul 9, 2025

3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization
Published on: February 18, 2022
Self-decorating cells via surface-initiated enzymatic controlled radical polymerization
Andrea Belluati1,2,3, Dominic Happel1, Malte Erbe1
1Department of Chemistry, Technical University of Darmstadt, Peter-Grünberg-Straße 4, 64287 Darmstadt, Germany. andrea.belluati@tu-darmstadt.de.
Abstract:
Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering.
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