Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

25.4K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
25.4K
What is Genetic Engineering?00:49

What is Genetic Engineering?

74.2K
Overview
74.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association of electrocardiographic left ventricular hypertrophy with cardiovascular events in hypertensive patients with and without chronic kidney disease.

Hypertension research : official journal of the Japanese Society of Hypertension·2026
Same author

Multinucleation and cytotoxicity induced by multikinase inhibition in highly proliferative cells.

Journal of toxicologic pathology·2026
Same author

Correction: Sphingosine-1-phosphate receptor 1/5 selective agonist alleviates ocular vascular pathologies.

Scientific reports·2026
Same author

Starch Biosynthesis-Related Genotype of Rice (<i>Wx</i> and <i>Alk</i>): Indicator of Suitability for <i>Sake</i> and <i>Shochu</i> Brewing Correlated with Water Absorption Ratio.

Journal of applied glycoscience·2026
Same author

Cross-species molecular mapping of the photoreceptor sensory cilium and periciliary complexes identifies conserved and species-specific architectural features.

bioRxiv : the preprint server for biology·2026
Same author

"Curtain sign" in lung ultrasonography: several different definitions.

Journal of anesthesia·2026

Related Experiment Video

Updated: Jul 9, 2025

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.6K

RNA-based controllers for engineering gene and cell therapies.

Kei Takahashi1, Kate E Galloway1

  • 1Department of Chemical Engineering, MIT, 25 Ames St., Cambridge, MA 02139, USA.

Current Opinion in Biotechnology
|December 5, 2023
PubMed
Summary

Engineered RNA controllers offer precise gene regulation and can be integrated into viral vectors for therapies. These compact, programmable RNA tools enhance safety and efficacy in gene and cell-based treatments.

More Related Videos

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.4K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

246

Related Experiment Videos

Last Updated: Jul 9, 2025

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

1.6K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.4K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

246

Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Gene Therapy

Background:

  • Engineered RNA-based genetic controllers offer compact and tunable post-transcriptional gene regulation.
  • The small size of RNA devices allows for portability to DNA and RNA viral vectors.
  • Recent advancements enable RNA tools to read and edit endogenous RNAs for transcriptional state analysis and programming.

Purpose of the Study:

  • To review RNA-based controllers and their potential applications.
  • To highlight their use in user-guided and autonomous systems for gene and cell-based therapies.

Main Methods:

  • Review of current literature on engineered RNA controllers.
  • Analysis of RNA tool capabilities for gene regulation, profiling, and programming.
  • Discussion of portability to viral vectors and integration into therapeutic strategies.

Main Results:

  • RNA controllers provide compact, tunable, and portable gene regulation.
  • Expanded RNA tool capabilities include reading and editing endogenous RNAs.
  • RNA-based controllers offer modularity, programmability, and enhanced safety, efficacy, and performance.

Conclusions:

  • RNA-based controllers are versatile tools for advanced gene and cell-based therapies.
  • Their compact and programmable nature supports greater control and customization.
  • Future applications include user-guided and autonomous therapeutic systems.