LncRNA H19 Influences Cellular Activities via the miR-454-3p/BHLHE40 Axis in Anaplastic Thyroid Carcinoma

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Updated: Jul 9, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
00:07

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.3K

Yang Wu1, Jihua Yang2, Honglai Zhang3

  • 1Department of General Surgery (Thyroid Gland/Blood Vessel), The First People's Hospital of Neijiang, Neijiang, China.

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|December 5, 2023
PubMed

Insights

Long non-coding RNA H19 (H19) promotes anaplastic thyroid carcinoma (ATC) by downregulating miR-454-3p and upregulating BHLHE40. This H19-miR-454-3p-BHLHE40 axis offers a potential therapeutic target for ATC.

Area of Science:

  • Molecular Oncology
  • Cancer Biology

Background:

  • Anaplastic thyroid carcinoma (ATC) is an aggressive malignancy with limited therapeutic options.
  • Previous research indicated that inhibiting long non-coding RNA H19 (lncRNA H19) suppresses ATC growth and metastasis.

Purpose of the Study:

  • To elucidate the underlying molecular mechanism of H19 in the progression of ATC.
  • To investigate the interaction between H19, miR-454-3p, and BHLHE40 in ATC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for gene expression analysis.
  • Dual-luciferase reporter assay and Pearson correlation analysis to determine molecular interactions.
  • Cellular assays (CCK-8, Transwell) and Western blot to assess functional impacts and protein levels.

Main Results:

  • H19 expression was significantly elevated in ATC tissues and cell lines.
  • H19 negatively regulated miR-454-3p, which was downregulated in ATC.
  • BHLHE40 mRNA was upregulated in ATC, inversely correlated with miR-454-3p, and positively correlated with H19.
  • H19 inhibition's effects on proliferation, migration, and invasion were reversed by miR-454-3p downregulation or BHLHE40 upregulation.
  • H19 modulated BHLHE40 to impact PI3K/AKT and apoptosis-related protein levels.

Conclusions:

  • The lncRNA H19-miR-454-3p-BHLHE40 axis plays a critical role in ATC pathogenesis.
  • This molecular pathway represents a potential therapeutic target and prognostic marker for ATC patients.

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