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Updated: Jul 9, 2025

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
Published on: November 1, 2017
Complete holography-based system for the identification of microparticles in water samples
Andouglas Gonçalves da Silva Júnior1,2, Cosimo Distante3, Luiz Marcos Garcia Gonçalves2
1Federal Institute of Rio Grande do Norte, Campus Parelhas, Rio Grande do Norte, Brazil.
Abstract:
Here, we present a comprehensive holography-based system designed for detecting microparticles through microscopic holographic projections of water samples. This system is designed for researchers who may be unfamiliar with holographic technology but are engaged in microparticle research, particularly in the field of water analysis. Additionally, our innovative system can be deployed for environmental monitoring as a component of an autonomous sailboat robot. Our system's primary application is for large-scale classification of diverse microplastics that are prevalent in water bodies worldwide. This paper provides a step-by-step guide for constructing our system and outlines its entire processing pipeline, including hologram acquisition for image reconstruction.

